DNA

Part:BBa_K3682003:Design

Designed by: Yu-Fan,Lin   Group: iGEM20_NYMU-Taipei   (2020-10-15)


RFP sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since we needed his tag to let our protein bind to our PolyHEMA gel, we added a TEV cleavage site and 6xhistag behind the original RFP. This is done by enclosing the original RFP sequence into a bacterial expression vector that has the two sequences above. We think this may be a great improvement in the existing RFP parts in iGEM registry since there may be other teams needing to use a his-tagged RFP. Also, the TEV cleavage site may alow the part users to cut off the 6xhistag whenever they needed.


Fig1. RFP with TEV and Histag in bacterial expression vector.

Source

We got the accessible RFP from a senior in our lab. The 6X histag and TEV cleavage site comes from the plasmid we bought at addgene.org(id 145730)

References